PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar
August Krogh Mini-Symposium on Regulatory Mechanisms in Muscle Metabolism
August Krogh Club
Single fiber proteomics as a magnifying glass for muscle plasticity
Assoc. Prof. Marta Murgia
University of Padova, Italy
Summary
The talk will give an overview of how inactivity and unloading modify the skeletal muscle proteome in a fiber type-specific manner.
We will discuss what bed rest and spaceflight have in common and which methodological approaches will lead to deeper knowledge of muscle fiber diversity.
August Krogh Club
The Path to Insulin Resistance
Prof. David James
University of Sydney, Australia
Summary
Insulin resistance represents a defect in the ability of insulin to regulate glucose metabolism with notable defects in insulin regulated glucose uptake into muscle cells.
It is critical to dissect the mechanistic basis of insulin resistance as it is one of the earliest defects that contributes to a series of diseases that comprise the metabolic syndrome. One of the conundrums has been that several disparate factors have been implicated in cellular insulin resistance including lipids like ceramides or diacylglycerides, mitochondrial defects and reactive oxygen species (ROS).
We have previously shown that insulin resistance is associated with lower CoQ levels in mitochondria and that this contributes to increased mitochondrial ROS and insulin resistance. We have obtained new evidence linking some of these factors together in what we refer to as an Insulin Resistance Pathway.
August Krogh Club
Insulin resistance in a dish: not about insulin signalling
Prof. Amira Klip
University of Toronto, Canada
Summary
Insulin resistance in vivo is a complex trait, but it is typically scored by diminished and delayed removal of dietary glucose from the blood.
This function is primarily dictated by insulin-induced uptake of dietary glucose into skeletal muscle, a phenomenon underlied by reduced insulin-dependent mobilization of GLUT4 to the plasma membrane.
The majority of studies to date have searched for defects in insulin signalling to understand the molecular underpinnings of insulin resistance. In the reductionary system of muscle cell culture, one can study insulin-derived signals and the defining step of GLUT4 translocation to the membrane.
Moreover, this step can be rendered insulin-resistant by incubating the cells with saturated fatty acids. In this muscle-cell autonomous insulin resistance model, we recently observed that:
i) the perinuclear GLUT4 compartment storage is dispersed compared to its compact location in insulin-sensitive cells;
ii) the cortical actin cytoskeleton is unresponsive to stimuli; and iii) upon insulin stimulation, surface GLUT4 levels do not rise as in control cells.
We will discuss how these alterations in basal-state distribution of GLUT4 and loss of actin filament dynamics arise, predisposing the cells to respond sub-optimally to insulin.
August Krogh Club
Identification of a genetic health variant that protects from metabolic disease
Senior Research Fellow, PhD. Jacqueline Stoeckli
University of Sydney, Australia
Summary
Metabolic diseases like type 2 diabetes are caused by a complex interaction between genetics and the environment. We searched for ‘health’ genes by studying a genetically diverse panel of outbred mice fed two different diets (chow versus western diet).
By classifying mice based on metabolic health, we identified a genome wide significant quantitative trail locus that was associated with metabolic health. Using systems genetics largely involving genetic analysis of tissue specific proteomes in a subset of mice, we pinpointed a causal gene at this locus.
August Krogh Club
New insights of AMPK function in skeletal muscle by integrative omics
Prof. Zhen Yan
University of Virginia, USA
Summary
Using a combination of acute and chronic endurance exercise training in genetically modified AMPKalpha2 (T172A) and Ulk1 (S555A) knock-in mice with phosphoproteomics and metabolomics, we have improved our understanding of the role of AMPK-Ulk1 regulatory exis in striated muscle adaptation in response to exercise training, indicating the importance of AMPK activation in glycolysis control during exercise.
Time
30 June 2023 12:00-16:00 CET (snacks and drinks will be served after the seminar)
Venue
Auditorium 1, August Krogh Building, Universitetsparken 13, DK-2100 Copenhagen
Registration
Participation is free, but please register here.
For PhD students
PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar
Contact
Jens Frey Halling, jefh@nexs.ku.dk
Kate Aiko Wickham, kawi@nexs.ku.dk
