Seminar: Muscle fiber types: from candidate transcript/protein analyses to global omics approaches

August Krogh Club triple Seminar

14:00-14:40: Professor Stefano Schiaffino: "Muscle fiber types: from candidate transcript/protein analyses to global omics approaches"
14:40-15:20: Assistant Professor Marta Murgia: “Exploring skeletal muscle plasticity and age-related changes by single fiber proteomics” 
15:20-16:00: Associate Professor Atul Shahaji Deshmukh: “Deep proteome and secretome analysis of skeletal muscle”
16:00-17:00: Post seminar servings and socializing.

Muscle fiber types: from candidate transcript/protein analyses to global omics approaches

v/ Professor Stefano Schiaffino, Venetian Institute of Molecular Medicine (VIMM), Padova, Italy. Presently guest professor at Institute of Sports Medicine, Bispebjerg Hospital, Copenhagen, Denmark.

Abstract

I will first illustrate how the proteomics approach can i) provide a better profile of the protein composition of the different muscle fiber types compared to previous traditional approaches and ii) become a discovery tool.

I will then make the point that the potential of proteomics and other omics approaches is presently poorly utilized because most studies are confined to a number of genes/proteins already extensively characterized.

I will finally introduce the project of exploring the proteome profile of the myotendinous junction, a poorly investigated specialized domain of skeletal muscle fibers.

Publications

Schiaffino S et al. Mitochondrial specialization revealed by single muscle fiber proteomics: focus on the Krebs cycle. Scand J Med Sci Sports. 2015; 25 Suppl 4:41-8.

Ciciliot S et al. Muscle type and fiber type specificity in muscle wasting. Int J Biochem Cell Biol. 2013; 45:2191-9.

Edwards AM et al. Too many roads not taken. Nature 470: 163-5.

Stoeger T et al, Large-scale investigation of the reasons why potentially important genes are ignored. PLoS Biol 2018.

Research profile

Professor Emeritus of the University of Padova. Founder and Vice President (2000-17) of the Venetian Institute of Molecular Medicine (VIMM).  Director (1987-2010) of the CNR Center of Muscle Biology and Physiopathology in Padova.

A major focus in my research has been to define the heterogeneity of muscle cells in skeletal and cardiac muscle. We focused initially on myosin heavy chains and prepared a battery of specific antibodies, which led to the discovery of a novel fast-type myosin, called type 2X, and a corresponding fiber type.

In the course of these studies we serendipitously found that cardiac muscle cells are also heterogeneous in term of myosin composition, thus providing specific molecular markers that for the first time allowed to distinguish between muscle cell populations in atrial and ventricular myocardium and heart conduction tissue.

More recently, we focused on the mechanisms that control fiber type specification, using an in vivo transfection approach based on intramuscular injection of plasmid DNA to generate selective perturbations of different signaling pathways. We found that the calcineurin-NFAT pathway controls activity-dependent fiber type specification, while the Akt-mTOR pathway controls muscle growth.

Subsequent studies, in collaboration with Marco Sandri and Alfred Goldberg, showed that FoxO transcription factors control the two main pathways of protein degradation in muscle cells, the proteasomal and autophagic-lysosomal pathways.

More recent work deals with i) circadian rhythms in skeletal muscle, ii) single muscle fiber proteomics, iii) the role of the MRF4-MEF2 axis in the regulation of skeletal muscle growth.

I am presently working in the lab of Michael Kjaer and Abigail Mackey in Copenhagen on the human myotendinous junctions.

Venue

Auditorium 1, August Krogh Building, Universitetsparken 13, DK-2100 Copenhagen

Registration

Participation is free, but please register here.

For PhD students

PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar

Contact

Jonas Møller Kristensen, jmkristensen@nexs.ku.dk, phone +45 3533 4776

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