Seminar: Exploring skeletal muscle plasticity and age-related changes by single fiber proteomics

August Krogh Club triple Seminar

14:00-14:40: Professor Stefano Schiaffino: "Muscle fiber types: from candidate transcript/protein analyses to global omics approaches"
14:40-15:20: Assistant Professor Marta Murgia: “Exploring skeletal muscle plasticity and age-related changes by single fiber proteomics” 
15:20-16:00: Associate Professor Atul Shahaji Deshmukh: “Deep proteome and secretome analysis of skeletal muscle”
16:00-17:00: Post seminar servings and socializing.

Exploring skeletal muscle plasticity and age-related changes by single fiber proteomics

v/ Assistant Professor Marta Murgia, Department of Biomedical Sciences, University of Padova, Italy and Department of Proteomics and Signal Transduction, Max-Planck-Institute of Biochemistry, Germany.

Abstract

To perform their diverse tasks, skeletal muscles employ four different fiber types, one type 1/slow and three different type 2/fast fibers, which are multinucleated cellular units with different contractile and metabolic properties. We have measured the proteome of single mouse muscle fibers using a liquid chromatography/mass spectrometry-based workflow optimized for low abundant and high dynamic range samples.

Our results provided a system view of muscle fiber diversity, highlighting a mitochondrial specialization in different fiber types. We have more recently compared human muscle fibers from young and older physically active donors, to study the impact of aging without confounding effects of inactivity.

We detected changes in carbohydrate metabolism and sarcomere homeostasis which might underlie the ability of slow, but not fast, muscle fibers to maintain their mass during aging.

Publications

Murgia M, Toniolo L, Nagaraj N, Ciciliot S, Vindigni V, Schiaffino S, Reggiani C, Mann M. Single Muscle Fiber Proteomics Reveals Fiber-Type-Specific Features of Human Muscle Aging. Cell Rep. 2017 Jun 13;19(11):2396-2409.

Murgia M, Nagaraj N, Deshmukh AS, Zeiler M, Cancellara P, Moretti I, Reggiani C, Schiaffino S, Mann M. Single muscle fiber proteomics reveals unexpected mitochondrial specialization. EMBO Rep. 2015 Mar;16(3):387-95.

Research profile

Marta Murgia obtained her PhD from University of Padova (Italy) working on calcium homeostasis in the cytosol and in other cellular compartments. She then moved to the field of skeletal muscle under the supervision of Stefano Schiaffino, investigating signalling pathways and transcription factors controlling activity-dependent gene regulation.

She joined the laboratory of Matthias Mann at Max-Planck-Institute of Biochemistry (Germany) in 2012, with the goal of applying proteomics to single muscle fibers. The collaboration is ongoing and developing in various directions with different model systems, focusing on the idea that proteomics is the ideal tool to describe tissue heterogeneity.

Venue

Auditorium 1, August Krogh Building, Universitetsparken 13, DK-2100 Copenhagen

Registration

Participation is free, but please register here.

For PhD students

PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar

Contact

Jonas Møller Kristensen, jmkristensen@nexs.ku.dk, phone +45 3533 4776

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