PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar
AKC Seminar: A novel approach to quantitatively assess protein movements using isolated skeletal muscle fibres: consequences of glycogen utilisation
August Krogh Club Seminar
Associate Professor Robyn M. Murphy
Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia.
Abstract
A number of proteins are either known to or are speculated to be associated with glycogen granules in skeletal muscle. In order to further understand the potential role glycogen might play in regulating muscle function, we have examined anabolic and catabolic glycogen associated proteins, AMPK (beta isoforms) and the insulin and contraction responsive glucose transporter, Glut4 before and after glycogen utilizing contractions, in both rat and human skeletal muscle. Using a unique approach of isolated individual skeletal muscle fibres where the surface membrane has been mechanically removed, we have quantitatively demonstrated the dynamic nature of some of these proteins.
Relevant papers
- Murphy RM and Lamb GD. (2013) Important considerations for protein analyses using antibody based techniques: Down-sizing western blotting up-sizes outcomes. J Physiol, 591: 5823-5831
Murphy RM, Xu H, Latchman H, Larkins NT, Gooley PR and Stapleton D. (2012) Single fiber analyses of glycogen related proteins reveal their differential association with glycogen in rat skeletal muscle. Am J Physiol Cell Physiol, 303: C1146-1155
Xu H, Stapleton D, Murphy RM. (2015) Rat skeletal muscle glycogen degradation pathways reveal differential association of glycogen related proteins with glycogen granules. J Biochem Physiol, 71:267-280.
Research profile
Dr Robyn Murphy is Associate Professor in the Department of Biochemistry and Genetics, La Trobe University, Melbourne, Australia. She holds a teaching and research position. She completed her PhD at Deakin University, Burwood, in the area of Exercise Biochemistry in 2003.
Robyn serves on Council for the Australian Physiological Society, and held the role National Secretary from 2010-2013, a role where she served as the Scientific Program Chair for three national meetings of the Society. Robyn is on the Executive Committee of the International Research Group on Biochemistry of Exercise.
Robyn has published 55 papers and her overall research focus is skeletal muscle function in health and disease. Most human muscle research is confounded by skeletal muscle fibre heterogeneity. To overcome issues associated with that, Robyn has pioneered methodology that allows specific proteins in skeletal muscle to be analysed quantitatively at the level of single muscle fibres. She has presented numerous invited seminars at national and international events discussing this aspect of her research, and has had many visitors to her laboratory learning the techniques.
Her biochemical methodologies can be uniquely coupled with physiological measurements in the same fibres, thereby providing mechanistic insights into muscle function. Robyn has particular interests in calcium dependent processes occurring in muscle as well as aspects of glucose regulation in health and disease, with a focus on glycogen and its associated proteins.
Time
23 June 2015
14:00-15:00: Seminar and discussion
15:00-15:30: Post seminar servings and socializing
Venue
Auditorium 1, August Krogh Building, Universitetsparken 13, DK-2100 Copenhagen
Registration
Participation is free, but please register here.
For PhD students
PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar
Contact
Christian Frøsig, CFrosig@nexs.ku.dk, mobile +45 2875 1617
