Jonas Roland Knudsen – University of Copenhagen

Jonas Roland Knudsen

Subcellular dynamics of mTORC1 and AMPK signaling in muscle

During my Master's Project I developed and characterized a method to isolate individual live muscle fibers from Extensor Digitorum Longus (EDL) muscles from mice at the section for Molecular Physiology (NEXS). In addition, I completed a three months research stay at National Institute of Health (NIH), United States, were I learned to perform electroporations and isolate individual muscle fibers from Flexor Digitorum Brevis (FDB) muscle. During my stay, I obtained data regarding the dynamics of beta actin in the muscle fibers, which called for further examination. Thanks to the August Krogh Summer Scholarship I got the opportunity to return to NIH to perform some additional experiments. This led to new findings regarding the dynamics of the beta actin cytoskeleton in muscle fibers and my results are now going to be included in a paper dealing with the role of beta actin cytoskeleton in insulin stimulated glucose transport in mature mouse muscle.

In addition this Summer Scholarship gave me the opportunity to establish the electroporation method at the section for Molecular Physiology and test some novel FRET biosensors with the aim to investigate the subcellular dynamics of mTORC1 and AMPK signaling.

Alongside my projects, I have applied for PhD funding together with my supervisor Thomas Jensen. The financial needs were fulfilled in late 2015 and the August Krogh Summer scholarship has therefore likely contributed to give me the opportunity to continue to do research every day, and for that I am deeply grateful.