PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar
AKC / CWS Double Seminar: Muscle cell chemoattraction of monocytes: effect of lipids and muscle contraction
August Krogh Club Double Seminar
Professor Amira Klip
14:00-15:00: Professor Sergio Grinstein seminar and discussion
15:00-16:00: Professor Amira Klip seminar and discussion
16:00-16:30: Reception and socializing
Muscle cell chemoattraction of monocytes: effect of lipids and muscle contraction
v/ Professor Amira Klip, Senior Scientist, The Hospital for Sick Children, and Professor of Paediatrics, Biochemistry and Physiology, The University of Toronto, Canada
Abstract
During exercise, muscle tissue gains macrophages originating from circulating monocytes, which help with tissue repair. These monocytes develop an anti-inflammatory phenotype. In contrast, during obesity, muscle tissue also gain macrophages but in this case they develop a pro-inflammatory phenotype. Using a reductionist system of cells in culture, we found that electrically-stimulated skeletal myotubes chemoattract monocytes, through the release of CCL2.
Conversely, myotubes treated with saturated fatty acids release nucleotides that act as the chemoatracting signal, and such myotubes confer a pro-inflammatory phenotype to macrophages. In vivo, monocytes must cross the microavscular endothelial barrier to reach the muscle fibers, and saturated fats cause increased adhesion and transmigration of monocytes across a microvascular endothelial cell monolayer. The microvasculature is also the barrier regulating insulin transfer from blood to muscle, and fatty acid treatment of microvascular endothelial cells reduces insulin transcytosis.
Relevant papers
- Pillon NJ, Li YE, Fink LN, Brozinick JT, Nikolayev A, Kuo MS, Bilan PJ, Klip A. (2014)
Nucleotides released from palmitate-challenged muscle cells through pannexin-3 attract monocytes.
Pillon NJ, Azizi PM, Li YE, Liu J, Wang C, Chan KL, Hopperton KE, Bazinet RP, Heit B, Bilan PJ, Lee WL, Klip A. (2015) Palmitate-induced inflammatory pathways in human adipose microvascular endothelial cells promote monocyte adhesion and impair insulin transcytosis.
Research profile
Dr. Amira Klip is a senior scientist in the Cell Biology Program at the SickKids Research Institute and Professor of Paediatrics, Biochemistry, and Physiology at the University of Toronto. In order to understand the process of insulin resistance, Dr. Klip studies insulin action and the cellular and molecular steps involved in this process. The work reveals a crosstalk between muscle and immune cells that contributes to insulin resistance.
Dr. Klip's lab has also established cellular platforms to investigate muscle contraction. She directs a laboratory of eight graduate students/postdoctoral fellows, one technician, and one research associate and has generated cell lines that are used by pharmaceutical companies to screen and identify potential anti-diabetic drugs.
Time
19 November 2015
14:00-15:00: Professor Sergio Grinstein seminar and discussion
15:00-16:00: Professor Amira Klip seminar and discussion
16:00-16:30: Reception and socializing
Venue
Auditorium 1, August Krogh Building, Universitetsparken 13, DK-2100 Copenhagen
Registration
Participation is free, but please register here.
For PhD students
PhD students participating in August Krogh seminars receive 0,2 ECTS per seminar
Contact
Christian Frøsig, CFrosig@nexs.ku.dk, mobile +45 2875 1617
